Annual UD CYTO Workshop
An annual “An Introduction to Flow Cytometry & Single Cell Technologies” 2-day short course & workshop (Part I or II and more) is offered by the core every year (Fall) and will be organized depending on core personnel availability. It is especially designed for new users who wish to use the flow cytometry or single-cell technologies at the core to analyze samples and can be paired with the 3-4 supervised flow training and/or sample preparation discussion sessions. This is a free course. We intend to offer the lectures as an introduction to flow cytometry & single-cell technologies for the UD and academic campuses. Core personnel will send out notifications on when we are ready to organize the workshop and will include the following topics with possible additions or changes as needed:
Part I – “An Introduction to Flow Cytometry: A Beginners’ Journey”
~6 hours (10 am to 12 pm > Lunch < 12:30 pm – 2:30 pm < Break > 3 pm – 5 pm)
– In-Person Presentation –
- What can flow cytometry tell you about your research question?
- Basic principles of flow cytometry and instrument operation.
- Introduction to respective instruments, software & shared equipment
- Overview of common applications and typical fluorescent stains
- Experimental Design, Single or Two Parameter Assay setup & Day of sample run
- Single- and Two-Color Flow Cytometry (& Sorting) is Simple!
- Multicolor (4 color-case study) Flow Cytometry and Color Compensation & Sorting
Part II – “An Introduction to Analysis, Single Cell Core and Methodologies”
~6 hours (10 am to 12 pm > Lunch < 12:30 pm – 2:30 pm < Break > 3 pm – 5 pm)
- 10-12-color T-cell analyses panel via FlowJo and tSNE
- Possibilities of a highly complex data set? – High Dimensional Spectral Cytometry
- Single Cell Methodologies at the core – Single Cell Core
- BD Rhapsody HT
- 10X Genomics Chromium X
- Parse Evercode WTA
- Others – Fluent Biosciences, IlluminaSequencing Options at UD Genomics Core (with Brewster Kingham)
- Bioinformatics analyses at the UD Bioinformatics Core (with Shawn Polson)
The following 3 days of the week are usually taken up by vendors, hands-on workshops and sample preparation and/or live demonstrations on our instruments.
Below are some excerpts from previous years!
